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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Immunol Methods. 2013 Jul 11;395(0):83–87. doi: 10.1016/j.jim.2013.06.010

Figure 1. Specificity test of the polyclonal scFvs populations by flow cytometry.

Figure 1

The polyclonal antibodies (pAb) selected using Lightning-Link® biotin-conjugated antigens (LL-Ags) show cross-reactivity with all the other LL-Ags – including LL-Ubi –, while the pAb obtained with ubiquitin conjugated with a different procedure are specific for their target even when biotinylated using a different procedure.