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The isolated monoclonal LL-Bind antibody shows cross-reactivity for all the LL-Ags but not for the antigen biotinylated with a different procedure (TF-Ubi, Avi-Ubi). The other clones in the top 10 ranking for all the LL-Ags selections show reactivity for their specific target, indicating that the cause of the apparent total cross-reactivity of the entire selected yeast population can be the presence of a single cross-reactive clone.
