. Author manuscript; available in PMC: 2014 Jan 13.

Published in final edited form as: Arch Biochem Biophys. 2011 Mar 30;510(1):53–61. doi: 10.1016/j.abb.2011.03.013

Table 3.

Analysis of HbS treated with NO by isoelectric focusing gel electrophoresis.^a

Hemolysate	Treatment condition	HbS concentration (μM)	HbS concentration (μM) at pI 7.4
HbS	0% O₂, 80 ppm NO	65	10.05 (15.5%)^b
HbS	3.5% O₂, 80 ppm NO	65	16.80 (25.9%)^b
HbS	21% O₂, 80 ppm NO	65	20.87 (32.1%)^b

HbS hemolysates (65 μM) were treated with 80 ppm NO in 0.1 M potassium phosphate buffer, pH 7.4, for 30 min to facilitate the interactions between Hb and NO.

Numbers in parentheses indicate percentages of modified HbS with NO relative to total HbS.