Skip to main content
. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Gastroenterology. 2013 Oct 9;146(1):157–165.e10. doi: 10.1053/j.gastro.2013.10.008

Figure 4. Nkx2-5 is required for the development and maintenance of the dorsal pyloric OLM.

Figure 4

Immunofluorescence of (i–iii) Nkx2-5flox/flox (WT) or (iv–vi) CAGGCre-ER™;Nkx2-5flox/flox (N25 CKO) pylorus (after two days of intraperitoneal tamoxifen injections) harvested at (A) E16.5 or (B) E18.5: (i,iv) DAPI; (ii,v) αSMA; or (iii,vi) merged. (A) In N25 CKO mice at E16.5, cell mass and αSMA expression is reduced in the dorsal OLM (asterisks). (B) At E18.5, the dorsal OLM in WT mice has expanded further and shows increased αSMA expression (Bi–iii). Deletion of Nkx2-5 beginning at E16.5, when the dorsal OLM is already αSMA positive (Aii–iii), leads to its regression (Biv–vi). Stomach is left; duodenum is right; and, dorsal is top. Green lines mark the epithelial basement membrane, and white lines separate ICM and OLM. Asterisk = dorsal pyloric OLM. Scale bars = 100 μm.