Skip to main content
. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Gastroenterology. 2013 Oct 9;146(1):157–165.e10. doi: 10.1053/j.gastro.2013.10.008

Figure 6. Regulatory hierarchy among Nkx2-5, Gata3 and Sox9 in the dorsal pyloric OLM.

Figure 6

Immunofluorescence of (A) WT, (B) CAGGCre-ER™;Nkx2-5flox/flox (N25 CKO), or (C) ) Gata3lacZ/lacZ (Gata3 KO) pylorus at E16.5: (i) DAPI; (ii) SOX9; (iii) NKX2-5; (iv) GATA3; or (v) merged. (A–C) Conditional loss of Nkx2-5 effectively eliminates dorsal OLM NKX2-5 expression (Biii), with concomitant loss of SOX9 expression (Bii), but does not abrogate GATA3 expression (Biv). Germline deficiency of Gata3 is associated with the complete absence of dorsal OLM SOX9 expression (Cii), but NKX2-5 is expressed in rare remaining dorsal OLM cells (Ciii). Stomach is left; duodenum is right; and, dorsal is top. Green lines mark the epithelial basement membrane, and white lines separate ICM and OLM. Scale bars = 100 μm.