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. Author manuscript; available in PMC: 2014 Jan 13.
Published in final edited form as: Traffic. 2012 Aug 5;13(11):1450–1456. doi: 10.1111/j.1600-0854.2012.01401.x

Figure 3. BRET is amenable to large-scale experments.

Figure 3

A) BRET signals observed between β2AR-Rluc8 or β2AR W158A-Rluc8 and acceptor proteins localized in the ER, Golgi (GA), PM, early endosomes (EE), late endosomes (LE) and recycling endosomes (RE), with or without overnight treatment with 10 μM alprenolol. Data represent mean ± s.d. (n = 4–11); *, P<0.01, unpaired t-test. Insets show confocal images of cells expressing β2AR-V or β2AR W158A-V; scale bars, 10 μm. B) The ratio of BRET observed with a PM acceptor to that observed with an ER acceptor in cells expressing β2AR W158A-Rluc8. Cells were incubated overnight with vehicle (DMSO) or a library of 83 adrenergic ligands and metabolites (at 10 μM). Data represent mean ± s.d. (n = 4).