Table 1.
Molecular assays tested in order of their target
| Target | Assay name and description (short name used in article) | Assay manufacturer (reference) |
|---|---|---|
| IS6110 | Exptl RT-PCR: 5 μl DNA input and Roche master mix (in-house Roche-5) | Roche Diagnostics Nederland BV, Almere, the Netherlands (21) |
| IS6110 | In-house RT-PCR IS6110: 5 μl DNA input and TaqMan universal PCR master mix, +AmpErase UNG (in-house TaqMan-5) | Applied Biosystems, Nieuwerkerk aan de Ijssel, the Netherlands (21) |
| IS6110 | In-house RT-PCR IS6110: 10 μl DNA input and TaqMan universal PCR master mix, +AmpErase UNG (in-house TaqMan-10) | Applied Biosystems (21) |
| IS6110 | MTB Q-PCR Alert kit detection of Mycobacterium tuberculosis DNA (Lucron) | Lucron ELITechGroup, Dieren, the Netherlands |
| IS6110 | RealAccurate Mycobacterium tuberculosis PCR kit (PathoFinder) | PathoFinder BV, Maastricht, the Netherlands |
| IS6110 | MTB real-time kit for detection of Mycobacterium tuberculosis complex (MP MTB kit) | Sacace Biotechnologies, Como, Italy, provided by MP Products, the Netherlands |
| IS6110 | MTB/NTM kit: multiplex MTC real-time PCR kit: AmpliSens MTC-FRT PCR kit (MP MTB/NTM) | InterLabService (ILS), Moscow, Russia, provided by MP Products, the Netherlands |
| IS6110 | Myco Direct 1.7, DNA based identification of Mycobacterium tuberculosis complex (MTUB) and other Mycobacteria (MOT) LCD array kit (Chipron)a | Chipron, GmbH, Berlin, Germany |
| 16S-23S rRNA gene | Internal transcribed sequence high-resolution melting (ITS-HRM) and Roche HRM master mix (in-house ITS-HRM) | Roche Diagnostics Nederland BV (11) |
| 16S rRNA of bp 584 | Cobas TaqMan MTB test CTM MTB (Cobas) | Roche Diagnostics Nederland BV |
| Direct repeat region of M. tuberculosis | Spoligotyping (spoligotyping)b | Homemade (22) |
| rpoB gene | GeneXpert MTB/RIF assay (GeneXpert) | Cepheid Benelux, Apeldoorn, the Netherlands (23) |
| Not disclosed by the manufacturer | AccuPower MTB real-time PCR kit (Goffin MTB) | Bioneer, South Korea, provided by Goffin, the Netherlands |
| Not disclosed by the manufacturer | AccuPower MTB&NTM real-time PCR kit (Goffin MTB/NTM) | Bioneer, South Korea, provided by Goffin |
a
The target-specific capture probes and the capture probes for the external control sequence Ctrl-EX1 together form a cross or a “+” sign.
b
Recorded positive if ≥3 spacers are present.