Table 2.

Intertest variability of assays targeting IS6110, calculated by standard deviation of the Cq values

Assay	Dilution	Result with IS6110 copy no. of:
		1	5	10	20
Expt 5a	Undiluted	0.19	0.29	0.06	0.07
	1:10,000	0.26	0.41	0.06	0.16
	ASb (1:10,000)	0.26	1.53	0.46	1.08
In-house TaqMan-5	Undiluted	0.04	0.39	0.04	0.01
	1:10,000	0.12	0.08	0.07	0.05
	AS	0.77	0.48	0.18	0.25
In-house TaqMan-10	Undiluted	0.07	0.05	0.02	0.13
	1:10,000	0.10	0.02	0.07	0.03
	AS	0.60	0.29	0.36	0.4
Lucron	Undiluted	0.05	0.03	0.04	0.26
	1:10,000	0.5	0.33	0.07	0.2
	AS	1.21	0.14	0.44	0.61
PathoFinder	Undiluted	0.03	0.01	0.08	0.02
	1:10,000	0.43	0.10	0.05	0.04
	AS	0.47	0.39	0.40	0.23
MP MTB	Undiluted	0.4	0.30	0.07	0.07
	1:10,000	0.09	0.22	0.16	0.10
	AS	0	0.06	1.03	0.23
MP MTB/NTMa	Undiluted	0.03	0.03	0.06	0.04
	1:10,000	3.2	1.00	0.07	0.04
	AS	0.57	0.27	0.69	0.39

^aAssays with highest intertest variability according to the standard deviation (SD).

^bAS, analytical sensitivity.