Table 3.
Intertest variability of assays not targeting IS6110, calculated by standard deviation of the Cq values
Assay | Dilution | Result with IS6110 copy no. of: |
|||
---|---|---|---|---|---|
1 | 5 | 10 | 20 | ||
In-house ITS-HRM | Undiluted | 0.04 | 0.06 | 0.06 | 0.06 |
1:10,000 | 0.25 | 0.61 | 0.64 | 0.15 | |
AS | 1.36 | 0.53 | 0.73 | 0.20 | |
Cobas | Undiluted | 0.1 | 0.25 | 0.1 | 0.06 |
1:10,000 | 0.25 | 0.2 | 0.31 | 0.21 | |
AS | 0.46 | 1.06 | 0.72 | 1.93 |