Table 3.
Results of the analysis of 122 isolates from 2000 by multiplex PCR rapid testing for detection of the M. tuberculosis Zaragoza strain and by spoligotyping
| Extraction and testinga | No. of positive isolates of: |
|
|---|---|---|
| M. tuberculosis | Zaragoza strain | |
| Extraction of genomic DNA | ||
| Multiplex PCR of the Zaragoza strain | 68 | 18 |
| Spoligotyping | 68 | 18 |
| Rapid DNA extraction | ||
| Multiplex PCR of the Zaragoza strain | 45b | 8c |
| Spoligotyping | 54 | 8 |
a
The two tests achieved sensitivities, specificities, PPVs, and NPVs of 100% in identifying M. tuberculosis Zaragoza isolates.
b
The absence of the gyrB amplicon in the rapid extraction samples could be due to degradation or insufficient amounts of DNA.
c
The 1,039-bp amplicon specific for the M. tuberculosis complex was not produced in one Zaragoza isolate.