Table 1.
Allelic diversity of the individual variable-number tandem-repeat loci and their different combinations in the panel of 115 Mycobacterium bovis isolates
| MIRU-VNTR typing analysis | No. of isolates with MIRU-VNTR allele no.: |
Dd | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | Nb | DBc | ||
| Individual MIRU-VNTR locus (alias) | ||||||||||||||||||
| QUB3232 (3232) | 2 | 6 | 17 | 32a | 30 | 7 | 11 | 5 | 1 | 1 | 2 | 1 | 0 | 0 | 0.8242 | |||
| ETR-A (2165) | 1 | 3 | 10 | 49 | 46a | 1 | 1 | 1 | 1 | 2 | 0.6438 | |||||||
| ETR-B (2461) | 9 | 17 | 76a | 11 | 1 | 0 | 1 | 0.5336 | ||||||||||
| QUB11a (2163a) | 1 | 2 | 1 | 10 | 1 | 6 | 83a | 1 | 3 | 1 | 1 | 5 | 0 | 0.4424 | ||||
| QUB26 (4052) | 2 | 6 | 11 | 89a | 2 | 1 | 2 | 1 | 0.3471 | |||||||||
| MIRU26 (2996) | 6 | 6 | 92a | 8 | 3 | 0 | 0.3450 | |||||||||||
| ETR-D (580) | 6 | 104a | 5 | 0 | 0 | 0.2038 | ||||||||||||
| ETR-E (3192) | 4 | 106a | 5 | 0 | 0.0807 | |||||||||||||
| QUB11b (2163b) | 3 | 109a | 1 | 2 | 0 | 0.0790 | ||||||||||||
| Combined MIRU-VNTR approaches | ||||||||||||||||||
| Four locie | 6 | 0.9689 | ||||||||||||||||
| Six locif | 8 | 0.9826 | ||||||||||||||||
| Nine locig | 12 | 0.9856 | ||||||||||||||||
a
Number of isolates at the allele that correspond to the most frequent MIRU-VNTR type.
b
N, number of isolates not amplifiable and therefore of undetermined MIRU-VNTR type.
c
DB, number of isolates with double bands.
d
D, discriminatory index.
e
Combination of QUB3232, ETR-A, ETR-B, and QUB11a.
f
Four-loci analysis plus QUB26 and MIRU26.
g
Six-loci analysis plus ETR-D, ETR-E, and QUB11b.