Table 2.
Systematic MIRU-VNTR typing of all Mycobacterium bovis SB0121 isolates (n = 176) from 15 farmsa
| Farm no. | No. of alleles at MIRU-VNTR locus (VNTR alias)b |
No. of isolates | Nature of outbreak (no. of types) | Other spoligotype(s) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| QUB3232 short (3232) | ETR-A (2165) | ETR-B (2461) | QUB11a (2163a) | QUB26 (4052) | MIRU26 (2996) | ETR-D (580) | ETR-E (3192) | QUB11b (2163b) | ||||
| 1 | 7 | 6 | 4 | 10 | 3 | 5 | 3 | 3 | 2 | 9 | Homogeneous | |
| 2 | 6 | 5 | 3 | 9 | 5 | 5 | 3 | 3 | 2 | 17 | Homogeneous | |
| 3 | 7 | 6 | 4 | 10 | 5 | 5 | 3 | 3 | 2 | 6 | Homogeneous | |
| 4 | 10 | 6 | 5 | 10 | 5 | 5 | 3 | 3 | 2 | 31 | Homogeneous | SB0339, SB0818, SB0140 |
| 5 | 4 | 5 | 4 | 10 | 5 | 5 | 3 | 3 | 2 | 7 | Homogeneous | |
| 6 | 7 | 6 | 4 | 10 | 6 | 5 | 3 | 3 | 2 | 5 | Heterogeneous (2) | |
| 7 | 6 | 4 | 10 | 5 | 2 | 3 | 3 | 2 | 4 | |||
| 7 | 7 | 6 | 4 | 10 | 5 | 5 | 3 | 3 | 2 | 11 | Heterogeneous (2) | SB0339, SB1142 |
| 7 | 5 | 3 | 7 | 5 | 5 | 3 | 3 | 2 | 1 | |||
| 8 | 7 | 6 | 3 | 10 | 4 | 5 | 3 | 3 | 2 | 29 | Heterogeneous (2) | SB0339 |
| 10 | 5 | 3 | 10 | 5 | 5 | 4 | 3 | 2 | 1 | |||
| 9 | 8 | 5 | 4 | 10 | 5 | 5 | 3 | 3 | 2 | 2 | Heterogeneous (3) | |
| 8 | 5 | 4 | 9 | 5 | 5 | 3 | 3 | 2 | 1 | |||
| 8 | 6 | 4 | 8 | 5 | 5 | 3 | 3 | 2 | 1 | |||
| 10 | 9 | 5 | 4 | 10 | 5 | 5 | 4 | 3 | 2 | 3 | Heterogeneous (3) | SB0295 |
| 10 | 5 | 3 | 10 | 5 | 5 | 3 | 3 | 2 | 1 | |||
| 7 | 6 | 4 | 10 | 2 | 2 | 3 | 3 | 2 | 1 | |||
| 11 | 9 | 3 | 3 | 10 | 5 | 5 | 3 | 3 | 2 | 9 | Heterogeneous (2) | |
| 9 | 5 | 3 | 10 | 5 | 5 | 3 | 3 | 2 | 6 | |||
| 12 | 15 | 5 | 4 | 9 | 5 | 5 | 3 | 3 | 1 | 6 | Heterogeneous (3) | SB0265, SB0807 |
| 11 | 5 | 4 | 9 | 5 | 5 | 3 | 3 | 1 | 1 | |||
| 8 | 12 | 5 | Nc | 4 | 4 | 3 | 3 | 2 | 1 | |||
| 13 | 10 | 5 | 4 | 10 | 5 | 5 | 3 | 3 | 2 | 6 | Heterogeneous (4) | SB0295, SB0416 |
| 6 | 5 | 4 | 12 | 4 | 6 | 3 | 3 | 2 | 1 | |||
| 6 | 6 | 4 | 10 | 5 | 5 | 3 | 3 | 2 | 1 | |||
| 7 | 5 | 4 | 10 | 5 | 6 | 3 | 3 | 2 | 1 | |||
| 14 | 6 | 5 | 2 | 7 | 5 | 5 | 3 | 3 | 2 | 3 | Heterogeneous (5) | SB0152, SB1320 |
| 8 | 6 | 4 | 10 | 5 | 5 | 3 | 3 | 2 | 1 | |||
| 6 | 6 | 4 | 10 | 5 | 5 | 3 | 3 | 2 | 1 | |||
| 7 | 5 | 4 | 10 | 4 | 5 | 3 | 3 | 2 | 1 | |||
| 7 | 6 | 4 | 10 | 5 | 5 | 3 | 3 | 2 | 1 | |||
| 15 | 7 | 6 | 4 | 10 | 6 | 5 | 3 | 3 | 2 | 2 | Heterogeneous (5) | SB0265, SB0875 |
| 7 | 5 | 5 | 10 | 5 | 5 | 3 | 2 | 2 | 2 | |||
| 14 | 6 | 4 | 13 | 4 | 6 | 3 | 3 | 2 | 1 | |||
| 8 | 5 | 5 | 10 | 5 | 5 | 3 | 2 | 2 | 1 | |||
| 7 | 6 | 3 | 7 | 5 | 5 | 3 | 3 | 2 | 1 | |||
a
This analysis revealed heterogeneity in 10 out of the 15 farms.
b
MIRU-VNTR loci with corresponding VNTR alias (markers have been ordered according to the D value).
c
N, not amplifiable.