Fig 1.

Schematic illustration of the MAS assay. The MAS assay starts with an allele-specific primer extension (ASPE) with all allele-specific primers mixed together in one reaction tube containing the reaction reagent mixture and a template. For the primer with a matching 3′-terminal nucleotide, primer extension occurs and biotinylated dCTPs are incorporated into the extended product. During the hybridization step, ASPE products are uniquely annealed to microspheres through the specificity of Tag/anti-Tag recognition. Finally, the hybridization products are read with the suspension array system, which identifies each microsphere set by its internal dye and records the associated reporter dye intensity as the mean fluorescence intensity (MFI).