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Detection of drug resistance mutations using plasmid DNA templates. After PCR amplification of each template individually, a mixture of the wild-type template and the mutant template (Mut) at a ratio of 1:1 (wt/wt), amplicons of each individual template, and a no-target negative control (NC) were analyzed with the 45 ASPE primers. The data shown are means plus standard deviations (SD) (error bars) (n = 5).
