Table 2.
Concordance between population sequencing and the MAS assay in DR mutation genotyping of patient specimens (n = 148) at each resistance mutation site
| pol region | Position | No. (%) of alleles detected | No. (%) of specimens with concordant resultsa | No. of discrepancies | No. of additional mixturesb |
|---|---|---|---|---|---|
| rt | 41 | 145 (97.97) | 144 (99.31) | 1 | 0 |
| 65 | 148 (100.0) | 145 (97.97) | 3 | 3 | |
| 70 | 142 (95.95) | 141 (99.30) | 1 | 1 | |
| 74 | 144 (97.30) | 144 (100.0) | 0 | 0 | |
| 115 | 145 (97.97) | 145 (100.0) | 0 | 0 | |
| 151 | 143 (96.62) | 143 (100.0) | 0 | 0 | |
| 184 | 137 (92.57) | 134 (97.81) | 3 | 2 | |
| 219 | 142 (95.95) | 141 (99.30) | 1 | 0 | |
| 100 | 143 (96.62) | 143 (100.0) | 0 | 0 | |
| 101c | 147 (99.32) | 143 (97.28) | 4 | 2 | |
| 103 | 146 (98.65) | 139 (95.21) | 7 | 5 | |
| 106 | 148 (100.0) | 146 (98.65) | 2 | 2 | |
| 181c | 137 (92.57) | 135 (98.54) | 2 | 1 | |
| 188c | 143 (96.62) | 141 (98.60) | 2 | 2 | |
| 190c | 145 (97.97) | 140 (96.55) | 5 | 5 | |
| prt | 32 | 148 (100.0) | 148 (100.0) | 0 | 0 |
| 47 | 148 (100.0) | 148 (100.0) | 0 | 0 | |
| 76 | 140 (94.59) | 140 (100.0) | 0 | 0 | |
| 84 | 148 (100.0) | 148 (100.0) | 0 | 0 | |
| 90 | 148 (100.0) | 147 (99.32) | 1 | 1 | |
| Total | 2,887 (97.53) | 2,855 (98.89) | 32 | 24 |
a
The concordance rate equals the number of concordant specimens divided by the number of alleles detected.
b
Number of additional mixtures detected by the MAS assay.
c
Six untargeted alleles at four loci could not be detected because no ASPE primers were designed for them; these include one K101Q, Y181I, Y188H, G190S, and two Y188C.