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CMV drug resistance mutation testing approach. Long-range PCR, followed by library preparation and bar coding using the Fluidigm Access Array, is shown relative to the UL97 (A) and UL54 (B) genes and known drug resistance mutations across the genes (not drawn to scale). This approach produces 4 overlapping amplicons for UL97 and 13 for UL54, which were pooled and subjected to 454 pyrosequencing using the Roche GS Junior sequencer.
