Table 3.

Target-specific primers for Access Array PCR

Gene and primer name	Primer 1a	Primer 2b	Nucleotides	Amino acids
UL54
Pol_D23	ACGCACACGGTGCAGGTACAGATCGTCGCG	GTGGAGCCCGTCAAGCTGGAGTT	604–880	950–1042
Pol_E44	CACCAGATCCACGCCCTTCATG	GGATTCGGAGGAGAGCAG	803–1078	884–975
Pol_F45	GCGTCAGGCCACGAAAGCGGAC	GTGCCGTGCGCGAATGCATGC	985–1355	792–915
Pol_F54	CGAAAGCGGACAAACACGCTGTC	GAATGCATGCGCGAGTGTCAAG	997–1343	796–911
Pol_G45	TGGGCAGACACGGCATCATG	CACGGTGTTTGAGCCCGAGG	1253–1613	706–825
Pol_G54	CGACCGATGCGCGTAATGCTG	AGGTGGGTTACTACAACGAC	1226–1596	712–834
Pol_H65	CACCAGCAGGGTGGAGTAGCAGAG	TGTGGCGGCTATGTTTCAGATGTC	1540–1807	641–730
Pol_I46	CGGCGCCGTGATTGTCGTTGG	CACCATTAATTTTCACTACGAG	1686–2055	559–681
Pol_I54	CTGGTACGAAACCGCCGCAGTAC	CGGACAGCAGATCCGTATCTAC	1662–1980	584–689
Pol_J56	GCAACACTATTCGTTTCGGGCAC	CCAAGGTGTATATTGCGGGTTC	1912–2287	481–606
Pol_K23	CTCGGCATTAGCCACGAAACAAC	GCAGCGCTTCTGCAAGTTGCCTAC	2163–2419	437–522
Pol_L11	ACCCACGGCGGGGCTGTGTAAA	GCTGCACTTCGGAGGGTGTGA	2399–2663	356–443
Pol_N22	GAATGACAATGTCATCGGACTTCTCGGCGCAG	GACCCGTGCCCGATTTGCAGTGTGTGTC	2795–3094	212–311
UL97
Pt_G45	CGTGCACCGCGGTCTGCTCAC	CGGCATGGGTCGGAAAGCAG	1194–1561	399–520
Pt_G65	CGGTACATCGACGTTTCCACAC	CGGCATGGGTCGGAAAGCAG	1248–1561	416–520
Pt_H22	CAGCCTCAGCGAGCCCTATCCGGATTAC	GTCGGAGCAGTGCGTGAGCTTGCCGTTC	1474–1787	492–596
Pt_I23	GACCGGCGCGGTCTGGACGAGGTGCGCATG	CCAACAGACGCTCCACGTTCTTTCTG	1735–1994	579–665

^aPrimer 1 is preceded by consensus sequence 1 (CS1) (ACACTGACGACATGGTTCTACA).

^bPrimer 2 is preceded by CS2 (TACGGTAGCAGAGACTTGGTCT).