. 2013 Nov;51(11):3720–3725. doi: 10.1128/JCM.01981-13

Table 1.

Primer sequences used for analysis of bacterial rRNA genes

Organism group	rRNA gene target	Real-time PCR primer set^a (5′–3′)	Pyrosequencing primer(s)^b (5′–3′)
Staphylococcus	16S	F^B, TGCCTAATACATGCAAGTCGAGCG; R, GTTGCCTTGGTAAGCCGTTACCTT	GTGTTACTCACCCGTCCGCCGCTA
Streptococcus	23S	F^B, GCCTTTTGTAGAATGAACCGGCGA; R, CGTTTGGAATTTCTCCGCTACCCA	TCACATGGTTTCGGGTCTA
Enteric	23S	F¹, CTAAGGCGAGGCCGAAAG; F², CTAAGGCGAGGCTGAAAAG; R^B, CTACCTGACCACCTGTGTCG	S¹, GGTTGTCCCGGTTTA; S², GGTCGTCCCGGTTCA
Universal	16S	F^B, AACTGGAGGAAGGTGGGGAT; R, AGGAGGTGATCCAACCGCA	TACAAGGCCCGGGAACGTATTCA

F^B, biotinylated forward primer; R, reverse primer; R^B, biotinylated reverse primer; F¹ and F², degenerate forward primers used in combination.

S¹ and S², degenerate pyrosequencing primers used in combination.