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. 2013 Nov;51(11):3760–3764. doi: 10.1128/JCM.01907-13

Fig 2.

Fig 2

Specificity of the RT-LAMP assay. Viral RNAs extracted from various control viruses and the reference virus were used. 1, H7N9 virus; 2, avian influenza A H7N1 virus; 3, avian influenza A H7N3 virus; 4, avian influenza A H7N7 virus; 5, avian influenza A H5N1 virus; 6, avian influenza A H9N2 virus; 7, human seasonal influenza A H3N2 virus; 8, 2009 swine-origin influenza virus A H1N1 virus; 9, human seasonal influenza A H1N1 virus; 10, HCoV-229E; 11, HCoV-OC43; 12, human rhinovirus N36; 13, influenza B virus; 14, adenovirus type 4; 15, parainfluenza virus type 3; NTC, no-template control. Detection by the HA-RT-LAMP (A) and NA-RT-LAMP (B) reactions was assessed by real-time turbidity assay using an LA-320c turbidimeter. Detection by the HA-RT-LAMP (C) and NA-RT-LAMP (D) reactions was also assessed by visible detection methods using hydroxynaphthol blue. The dashed gray lines in panels A and B and the red vertical dotted lines in panels C and D indicate the cutoffs for positive versus negative.