Table 1.
CMV test methodsb
| Test method, component, or characteristic | Lab 1 | Lab 2 | Lab 3 | Lab 4 | Lab 5 |
|---|---|---|---|---|---|
| Assay system | Lab-developed test | Qiagen Artus CMV (ASR) | Qiagen Artus CMV (ASR) | Lab-developed test | Luminex MultiCode CMV (ASR)a |
| Laboratory calibrators | LD (synthetic) | Qiagen Quant standards | Qiagen Quant standards | LD (synthetic) | LD (synthetic) |
| Gene target | Glycoprotein B (UL55) and IE-1 (UL123) | IE-2 (UL122) | IE-2 (UL122) | Glycoprotein B (UL55) | DNA polymerase (UL54) |
| Amplicon size (bp) | 64 (UL55), 76 (UL123) | 105 | 105 | 254 | 52 |
| Extraction method | MagNA Pure TNA kit; magnetic silica particles | MagNA Pure TNA kit; magnetic silica particles | Qiagen M48; MagAttract viral RNA; magnetic silica particles | QIAamp DNA minikit; column based; QIAcube | Qiagen M48; MagAttract DNA minikit; magnetic silica particles |
| Probe chemistry | TaqMan; cleaved probe | TaqMan; cleaved probe | TaqMan; cleaved probe | FRET; probes remain intact | Labeled primers; no probes |
| Detection system | ABI StepOnePlus | ABI Prism 7500 | ABI Prism 7500 | LightCycler 2.0 | ABI Prism 7500 |
a
Formerly produced by Eragen Biosciences.
b
LD, lab developed; ASR, analyte-specific reagent; FRET, fluorescent resonance energy transfer.