Fig 2.

Elution peaks obtained in the chromatographic runs involving samples 1 (stool, Benzonase, CIM one-step elution), 2 (cell culture, Benzonase, CIM one-step elution), and 3 (cell culture, CIM gradient elution). mAU, milliabsorbance units. Fraction analysis for 18S rRNA (as indicative of eukaryotic RNA presence) and reovirus EM detection is shown below the graphs. In the case of sample 1, the presence of reovirus had to be confirmed by classic quantitative RT-PCR (RT-qPCR) (shown to the left below). NTC, negative template control; NT, not tested; undet, undetermined.