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. 2013 Jul 3;22(22):4646–4652. doi: 10.1093/hmg/ddt313

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© The Author 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — (A) siRNA knockdown of JIP1 does not affect APP processing or calsyntenin-1 levels in rat cortical neurons. Neurons were treated with control (Ctrl) or JIP1 mix siRNAs (JIP1) and the samples probed on immunoblots for full-length APP (APP), APP phosphorylated on threonine-668 (pAPP), total sAPP, sAPPα and sAPPβ in conditioned media, and calsyntenin-1 (Cstn); actin is shown as a loading control. No significant changes in the levels of any of these proteins or phosphorylation of APP on threonine-668 were detected between control or JIP1-siRNA treated neurons (Student's t-test n = 3). (B) siRNA knockdown of calsyntenin-1 does not affect JIP1 protein levels. Neurons were treated with control (Ctrl) or calsyntenin-1 (Cstn) siRNAs and the samples probed on immunoblots for JIP1 and actin as a loading control. No significant changes in the levels of JIP1 were detected between control or calsyntenin-1 siRNA-treated neurons (Student's t-test n = 3).