Fig. 1.

a A tissue microarray was prepared containing 44 invasive ductal carcinoma of the breast. EP4 and H&E by immunohistochemistry. (i) Benign lobule, EP4, 1+; (ii) H&E; (iii) invasive ductal carcinoma, EP4, 1+; (iv) H&E; (v) invasive ductal carcinoma, EP4, 3+; (vi) H&E. b Line 410.4 tumor cells injected proximal to the mammary fat pad of Balb/cByJ female mice treated with vehicle or RQ-08 (30 mg/kg/day). When tumors measured 18 mm in diameter, mice were euthanized and surface lung tumor colonies enumerated. Mean ± SE, P = 0.04. c MDA-MB-231-luciferase cells treated with RQ-15986 (3.0 μM/l) or DMSO vehicle and injected i.v. into groups of five Balb/SCID mice and live animal imaging carried out at 5 min and at the days indicated. Data expressed as percent photons detected relative to day 0. d Line 66.1 cells transfected with plasmid expressing shEP4 or vector; stable clones were derived and EP4 expression characterized by qPCR. e Cell lines from d injected i.v. into 5–10 Balb/cByJ female mice and surface lung tumor colonies quantified. Mean ± SE, P < 0.01