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SDS-PAGE Aalysis of mg-MSDH. Lane M, codonplus; lane 2, BL 21-codonplus pET/mg-MSDH; BL 21-codonplus pET/mg-MSDH after IPTG induction; lane4, the supernatant of BL 21-codonplus pET/mg-MSDH; lane 5 the reconstruction mg-MSDH by Ni- NTA affinity chromatography
