Table 1.

The primers for PCR and walking

Primer name	For purpose	Primer sequence
F motif4	The first semi-nested PCR	5-TTHAGVGGHTGYBYBGW-3
F motif6	The second semi-nested PCR	5-GGBCARVKDTGYAWHK-3
R motif10	Semi-nested PCR	5-TTSHNGCCKCCRAARGG-3
DW-ACP 1	The first walking	5-ACP-AGGTC-3′
DW-ACP 2	The first walking	5-ACP-TGGTC-3′
DW-ACP 3	The first walking	5-ACP-GGGTC-3′
DW-ACP 4	The first walking	5-ACP-CGGTC-3′
DW-ACPN	The second walking	5-ACPN-GGTC-3′
Uni-primer	The first walking	5-TCACAGAAGTATGCCAAGCGA-3′
R-Tsp1	The first walking	5-TAAAAACGCCAGAGAGTTCT-3′
R-Tsp2	The second walking	5-CCTTCTTGTAGATGTGCCTGG-3′
R-Tsp3	The third walking	5-GCAATATAGGAGTCAACATAGG-3′
F-TSP1	The first walking	5-TCTATGTACCTCAGAACAGTGT-3′
F-TSP2	The second walking	5-AGGCTGCCTCGACAAGCG-3′
F-TSP3	The third walking	5-CTCGCCTGCGAGGCATCTCTG-3′
F-full	Full length sequence amplification	5-tctagaGTGTTAAATGGAGGATTAGAA-3′
R-full	Full length sequence amplification	5-ctcgagCCACCTGGATATGATGACCTTC-3′