Table 2.

Oligonucleotides used in this study

Oligonucleotide	Sequence (5′ → 3′)	Description
CiaB-DF	TAYACNCAYGGNGTNGC	Degenerate PCR
CiaB-DR	TCRTGNCCKATNGTNGA
CiaB-F	GTGAACGATTTTAAAAGATTAAACG	Strain PCR
CiaB-R	CTATCTCTCGCTCGCTTGCC
CiaB-RTF1	CTAGAACGCAAACCTACATCA	RT-PCR detection
CiaB-RTR1	CCGCTAAGCTTCATAAACGG
M13 F	CGCCAGGGTTTTCCCAGTCACGA	DNA Sequencing
M13 R	TCACACAGGAAACAGCTATGAC
CiaB-F5	CAAACTATAAAAATTTGGCGCTTC
CiaB-R5	GAAAAGCTTTGCGAAAGTCG
CiaB-Walk-5R	TACATCGCGTTTAGTTCGTCTTTGC	Genome Walker
CiaB-Walk-3F	CTATAGGAAACGAGCTTGACGAGAGC
AP1	GAAAAGCTTTGCGAAAGTCG
AP2	ACTATAGGGCACGCGTGGT

N A, T, G, or C; Y C or T; R A or G; K G or T (IUPAC nucleotide ambiguity codes)