Fig. 4.

CpG island shore regions showed age-related hypomethylation not only in muscle, but also in the liver and brain. The methylation levels of CpG island shore regions in EF1α1 (region e in Fig. 2), fli1a (region b in Fig. S3), and fgf3 (region b in Fig. S4) in the zebrafish muscle, liver, and brain were examined by bisulfite sequencing. *p < 0.05, **p < 0.01, and ***p < 0.001. Four fish per stage were analyzed. Horizontal bars show medians