Fig. 2.
The intracellular ROS levels in Bmi-1+/− brain cells. a The representative results showing ROS levels assessed by DCFDA fluorescence in Bmi-1+/+ and Bmi-1+/− brain cells under basal conditions, or without DCFDA staining (negative control), or following addition of 1 μM H2O2 treatment for 10 min (positive control). Geometric mean (Geo Mean) was used to calculate the total intensity of fluorescence. b The intracellular ROS levels in Bmi-1+/− brain cells did not show a great difference compared with wild type under basal conditions, but dramatically increased in response to H2O2 stimulation. Data represent mean ± SEM from five mice per group performed in triplicate. *P < 0.05 vs. Bmi-1+/+ mice