Table 2.

PCR primers and annealing temperature used for amplification of selected regions of DNMT1 gene

Gene	Region	PCR primers		PCR product size [bp]	Annealing temperature [°C]
DNMT1	Exon 3	F	CAAAGTTGCTTAACGAGTGTTTTT	821	58
		R	TAACGCTATCAAGTAACGGGAGA
	Exons 22–23	F	AAAAGAGGAAGATTGGCAACAG	672	60
		R	CTGCTCGAGAAAGGGTCTGG
	Exons 37–38	F	GTAGACAGTGTTAAGCGGCAAAAG	698	60
		R	ATGCCCACCTGGTCAGAG