Figure 7.

Granulocyte accumulation in Muc5ac^+/+ or Muc5ac^−/− during ventilator-induced lung inury (VILI). (a–h) Muc5ac^−/− mice or littermate controls matched in age, gender, and weight were mechanically ventilated (inspiratory pressure 45 mbar, inspired oxygen fraction 1.0, positive-end expiratory pressure 3 mbar), followed by collection of bronchoalveolar fluid (BAL) and harvest of the lungs. Flow cytometry was performed on tissue homogenates (a–d) and corresponding BAL (e–h) for eosinophils (EOS; SiglecF⁺/Ly6G⁻/CD11b⁺), polymorphnuclear neutrophils (PMN; Ly6G^high/CD11 b^high/SiglecF), dendritic cells (DC; MHCII⁺ CD11c^high), and macrophages (Mφ); F4/80⁺/MHCII⁺/CD11b⁺/Ly6G-) following gating of Live CD45⁺ leukocytes. (a) Absolute cell numbers from Muc5ac^−/− or Muc5ac^+/+ lung homogenate at baseline. (b) Representative flow cytometry for PMNs from Muc5ac^−/− or Muc5ac^+/+ lung homogenates at baseline. (c) Absolute cell numbers from Muc5ac^−/− or Muc5ac^+/+ lung homogenates after 3 h of mechanical ventilation (as described above). (d) Representative flow cytometry for PMNs from Muc5ac^−/− or Muc5ac^+/+ lung homogenates after mechanical ventilation (as described above). (e) Absolute cell numbers from Muc5ac^−/− or Muc5ac^+/+ BAL at baseline. (f) Representative flow cytometry for PMNs from Muc5ac^−/− or Muc5ac^+/+ BAL at baselines. (g) Absolute cell numbers from Muc5ac^−/− or Muc5ac^+/+ lung homogenates after 3 h of mechanical ventilation (as described above). (h) Representative flow cytometry for PMNs from Muc5ac^−/− or Muc5ac^+/+ BAL after mechanical ventilation (as described above). All data are expressed as mean ± s.d. (n = 5 per strain).