Table 2. Growth-phase regulated chromosomal expression of GFPuv in CVD 910 attenuated Salmonella Typhi live vectors.
| Time (hr) | CVD 910 | CVD 910-GG (guaBA::gfpuv) |
CVD 910-HG (htrA::gfpuv) |
CVD 910–2G (guaBA::gfpuv htrA::gfpuv) |
||||
|---|---|---|---|---|---|---|---|---|
| OD600a | MFI b | OD600 | MFI | OD600 | MFI | OD600 | MFI | |
| 0 | 0.04 | ND c | 0.04 | ND | 0.03 | ND | 0.02 | ND |
| 1 | 0.09 | ND | 0.09 | 74.94 | 0.06 | 32.9 | 0.06 | 38.31 |
| 2 | 0.33 | ND | 0.3 | 71.03 | 0.24 | 49.08 | 0.24 | 72.53 |
| 3 | 0.81 | ND | 0.72 | 70.58 | 0.68 | 56.12 | 0.6 | 95.41 |
| 4 | 1.45 | ND | 1.31 | 75.26 | 1.29 | 60 | 1.36 | 121.95 |
| 5 | 1.96 | ND | 1.86 | 84.81 | 1.84 | 66.55 | 1.86 | 138.01 |
| 6 | 2.24 | 5.87 | 2.17 | 96.31 | 2.19 | 68.94 | 2.16 | 161.65 |
aCultures grown under non-inducing conditions in 50 mM NaCl; bMean Fluorescence Intensity; cNot Determined