Table 3. Primers used in the construction and testing of live vector strains expressing chromosomally encoded GFPuv.

Primer	Sequencea
5guaBA-for	5′- GAA TTC TAG CTG CTC ATA CTT CTG CTG CA -3′
5guaBA-rev	5′- GCT AGC CAA TTG GGG CAA TAT CTC ACC TGG -3′
3guaBA-for	5′- GGA TCC ACT AGT GTC GAT AAC CCT TCC TGT GT -3′
3guaBA-rev	5′- CTC GAG ACA GCA CCT ACA AGT CTG GCA TG -3′
guaBA PCR-for	5′- GCG CTG ACC ACC GGA ATA CGG CTG -3′
guaBA PCR-rev	5′- CAT GGC ATG GAT GAG GCA ACC GCG AAG C -3′
5htrA-for	5′- GAA TTC GTA CCT TCA ATC AGG CGT TAC TGG AAG ATG -3′
5htrA-rev	5′- GCT AGC CAA TTG CGA TTA ACA GGT AAC GCA AAA TTG CTG TGT ACG TCA G -3′
3htrA-for	5′- GGA TCC ACT AGT CTG CGT AAG ATT CTC GAC AGC AAG CCG TCG GT -3′
3htrA-rev	5′- CTC GAG CCA GCA TCA TTT CGG CAG TCA TAC ACA CCA GTT CGC -3′
htrA PCR-for	5′-GTG TCG CCG ATC TTG AAG ACG CGG TAG AG -3′
htrA PCR-rev	5′- CTA TCG ACG CCA AGC TGG CCG CTG TCG AC -3
5clyA-for	5′- TAG TAA TGA GAA TTC GCT GGT ATT GAT CGG CTC TCC GGT AGA GAT TAG CGA -3′
5clyA-rev	5′- GCT AGC CAA TTG TGC CTC TTT AAA TAT ATA AAT TGC AAT TAA GTA CCT G -3′
3clyA-for	5′- GGA TCC ACT AGT GAT ACA TTT TCA TTC GAT CTG TGT ACT TTT AAC GCC CGA TAG CG -3′
3clyA-rev	5′- TGA TAG TAA CTC GAG ACA ATC CAT AAG AAA GGT CAG GCA CAC TGG GAA GGC GAC ATC -3′
clyA PCR-for	5′- CAT GAT GGT ATC CAG TAT GGC ACA AGC -3′
clyA PCR-rev	5′- GTA ATC GAC AAC ATG CTA CAT CCA TCG -3′
5FRT-aph-for	5′- GAA TTC GCT AGC GCT GGA GCT GCT TCG AAG TTC -3′
3FRT-aph-rev	5′- CTC GAG TTC CGG GGA TCC GTC GAC CTG CAG TTC -3′
5gfpuv	5′- CAA TTG TGT GGT AGC ACA GAA TAA TGA AAA GT -3′
3gfpuv	5′- GCT AGC TCA TTA TTT GTA GAG CTC ATC CAT -3′

^a Relevant restriction sites are underlined.