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. Author manuscript; available in PMC: 2014 Nov 5.
Published in final edited form as: Biochemistry. 2013 Oct 23;52(44):7840–7855. doi: 10.1021/bi401083b

Table 7.

Effects of mutating neighboring residue to Ala on the Tyr pKa

Residue mutated pKa ΔpKa
None 6.3 (0)
Phe56 6.9 0.6
Phe86 6.3 0
Met105 6.3 0
Met116 5.9 −0.4

None refers to Asp40Asn.