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. Author manuscript; available in PMC: 2014 Jan 13.
Published in final edited form as: Recent Pat Space Technol. 2013 Jun 1;3(1):40–47. doi: 10.2174/18776116112029990011

Figure 8. Time-line of activities for bone marrow macrophage differentiation on STS-126.

Figure 8

The FPAs were prepared as previously described [5] and were shipped to the NASA SLSL at KSC 2 days before launch. The FPAs were loaded into GAPs and placed at 37°C in the Commercial Generic Bioprocessing Apparatus (CGBA). Parallel temperature and activation profile conditions were maintained on GAPs kept at the SLSL. The cells in the FPAs were re-fed with 1.5 ml of medium supplemented with rmM-CSF on day 5 of the spaceflight by combining chambers 1 and 2 (See Figure 3). On day 14 of spaceflight, the content of the FPAs’ third chamber were mixed with the cell suspension in the previously merged chambers to preserve RNA or to fix or maintain cells until processing after landing. STS-126 landing occurred at the Edwards Air Force Base in California after 16 days of space flight. Samples were placed at 4° C and transported to the SLSL in Florida.