Table 1.
Recovery of cells from bioreactor
| Horizontal1 | Vertical1 | |||
|---|---|---|---|---|
| Rotation | Stationary | Rotation | Stationary | |
| Viable Cells Recovered2 | 1.6±0.3 × 107 | 3.6±0.5 × 106 | 3.2±0.5 × 106 | 1.1±0 × 106 |
| % Cell input Recovered3 | 5.3% | 1.2% | 1.1% | 0.4% |
1
3×108 primary mouse bone marrow cells were seeded in a chamber volume of 50 ml containing 15ng/ml mCSF.
2
Cells were incubated for 7 days. They were fed with 15 ml fresh medium on days 3 and 5.
3
% Cell input Recovered=(# of viable cells recovered/# viable cells added to the culture vessel)×100