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. 2014 Jan 13;9(1):e85172. doi: 10.1371/journal.pone.0085172

Figure 2. Mapping regulatory elements within the CHD5 promoter.

Figure 2

The reporter constructs containing the full length (–2000 to –1) CHD5 promoter or partial promoter sequences progressively truncated by 200 bp (A) or 100 bp (B) at the 5' or 3' end were measured for luciferase activity in K-562 cells. The promoterless pGL4.10-Basic vector was used as a negative control. The pGL4.10-CMV vector, which uses the CMV promoter, was used as a positive reporter construct. The relative Luc activity of full length and truncated CHD5 promoters were normalized to Renilla luciferase activity and compared to normalized Luc luciferase activity from pGL4.10-Basic. All data are presented as mean ± SD. P<0.001 was considered statistically significant (**).