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. 2014 Jan 13;9(1):e85172. doi: 10.1371/journal.pone.0085172

Figure 8. Identification of the AP2 binding site in the CHD5 promoter regulatory element.

Figure 8

ChIP-qPCR assay indicated that AP2 binds to the identified regulatory element of the CHD5 promoter (A, B). Normal IgG was used as a negative control. The pGL4.10-CHD5-2000 to -370 and pGL4.10-CHD5-356 vectors, in which the AP2 binding site was deleted, were measured for luciferase activity in K-562 cells (C). The pGL4.1-CHD5-P-CR vector, harboring the –560 to –240 region, was measured for luciferase activity in K-562 cells (D). ChIP-qPCR analysis showed that DAC treatment enhanced AP2 binding to the CHD5 promoter. All data are presented as mean ± SD. P<0.001 was considered statistically significant (**).