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. 2014 Jan 13;9(1):e85172. doi: 10.1371/journal.pone.0085172

Table 2. Primers for construction of reporter constructs.

Region of the deleted CHD5 promoter (upstream of TST) Primer Sequence
−1800bp to −1bp CHD5-PD1-F ATGAGCATCTCGGCTCTAATTTGTTG
CHD5-PD1-R AGATCTTGATATCCTCGAGG
−1600bp to −1bp CHD5-PD2-F TTGGGGGGCTGGTAACTGAG
CHD5-PD2-R AGATCTTGATATCCTCGAGG
−1400bp to −1bp CHD5-PD3-F GCCTGCTTCCCTGAAGAGTGTT
CHD5-PD3-R AGATCTTGATATCCTCGAGG
−1200bp to −1bp CHD5-PD4-F GGCAATAAAATTGAACGGGA
CHD5-PD4-R AGATCTTGATATCCTCGAGG
−1000bp to −1bp CHD5-PD5-F AGGCGCTCATTTAAAATTCC
CHD5-PD5-R AGATCTTGATATCCTCGAGG
−800bp to −1bp CHD5-PD6-F GGGCTTTTTGGAAGGGGGCA
CHD5-PD6-R AGATCTTGATATCCTCGAGG
−600bp to −1bp CHD5-PD7-F GTCCCGGCGCCTGTGAACCG
CHD5-PD7-R AGATCTTGATATCCTCGAGG
−400bp to −1bp CHD5-PD8-F TCGGGTCCGCGGGCGCGCGG
CHD5-PD8-R AGATCTTGATATCCTCGAGG
−200bp to −1bp CHD5-PD9-F CCGTGCCGCCGTGCCTCTGG
CHD5-PD9-R AGATCTTGATATCCTCGAGG
−2000bp to −200bp CHD5-PD10-F AAGCTTGGCAATCCGGTACTGTTGGTAAAGC
CHD5-PD10-R CGGGGGGGCGGCACACATGC
−2000bp to −400bp CHD5-PD11-F AAGCTTGGCAATCCGGTACTGTTGGTAAAGC
CHD5-PD11-R CGGCCGAGCGGGCAGTCGGG
−2000bp to −600bp CHD5-PD12-F AAGCTTGGCAATCCGGTACTGTTGGTAAAGC
CHD5-PD12-R CCACCCCGCTCCCGGCTCCG
−2000bp to −800bp CHD5-PD13-F AAGCTTGGCAATCCGGTACTG
CHD5-PD13-R CTCCAGGCTGGGTGCAGCTG
−2000bp to −1000bp CHD5-PD14-F AAGCTTGGCAATCCGGTACTGTTGGTAAA
CHD5-PD14-R CCAGTCTCCGCGGCGGCAAG
−2000bp to −1200bp CHD5-PD15-F AAGCTTGGCAATCCGGTACT
CHD5-PD15-R AGCAAACCCCAAACAAAAGA
−2000bp to −1400bp CHD5-PD16-F AAGCTTGGCAATCCGGTACT
CHD5-PD16-R CAGGAAACCAAAGGGCCTCC
−2000bp to −1600bp CHD5-PD17-F AAGCTTGGCAATCCGGTACT
CHD5-PD17-R CACCTCTCTAATCCCTTTCA
−2000bp to −1800bp CHD5-PD18-F AAGCTTGGCAATCCGGTACT
CHD5-PD18-R CAGTCATAATCCTCTCCCCA
−2000bp to −700bp and −600bp to −1bp CHD5-PD19-F GTCCCGGCGCCTGTGAACCG
CHD5-PD19-R AGACTGTGGGTCTGATTTAC
−2000bp to −700bp and −500bp to −1bp CHD5-PD20-F CGAGCGGCGGCGGCTGGCTC
CHD5-PD20-R AGACTGTGGGTCTGATTTAC
−2000bp to −700bp and −400bp to −1bp CHD5-PD21-F TCGGGTCCGCGGGCGCGCGG
CHD5-PD21-R AGACTGTGGGTCTGATTTAC
−2000bp to −700bp and −300bp to −1bp CHD5-PD22-F TTCTCTGCTGTTAACTAGTC
CHD5-PD22-R AGACTGTGGGTCTGATTTAC
−2000bp to −700bp and −200bp to −1bp CHD5-PD23-F CCGTGCCGCCGTGCCTCTGG
CHD5-PD23-R AGACTGTGGGTCTGATTTAC
−2000bp to −700bp and −100bp to−1bp CHD5-PD24-F GCAGGCTAAGGCGGCCGAGA
CHD5-PD24-R AGACTGTGGGTCTGATTTAC
−2000bp to −100bp CHD5-PD25-F AAGCTTGGCAATCCGGTACT
CHD5-PD25-R TCCCCGCAAAGCCCGGGCGC
−2000bp to −200bp CHD5-PD26-F AAGCTTGGCAATCCGGTACTGTTGGTAAAGC
CHD5-PD26-R CGGGGGGGCGGCACACATGC
−2000bp to −300bp CHD5-PD27-F AAGCTTGGCAATCCGGTACT
CHD5-PD27-R CAAGTTGGGGGACTAGTCCT
−2000bp to −400bp CHD5-PD28-F AAGCTTGGCAATCCGGTACTGTTGGTAAAGC
CHD5-PD28-R CGGCCGAGCGGGCAGTCGGG
−2000bp to −500bp CHD5-PD29-F AAGCTTGGCAATCCGGTACTGTTGGTAAA
CHD5-PD29-R CCTGCGAAGGCGCTGGGCCC
−2000bp to −600bp CHD5-PD30-F AAGCTTGGCAATCCGGTACTGTTGGTAAAGC
CHD5-PD30-R CCACCCCGCTCCCGGCTCCG
2000bp to −370bp and −356bp to −1bp CHD5-P-AP-2-D-F GGGCGCCCCCCGGTCCCCACC
CHD5-P-AP-2-D-R CGTCGAGGCGTCCGCGCG
–560bp to –240bp CHD5-P-CR-F GCTGCCCTGAAGACCCTG
CHD5-P-CR-R CTGGTTGCTGTGGCAACC