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. 2014 Jan 15;25(2):234–244. doi: 10.1091/mbc.E13-08-0453

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© 2014 Nakamoto et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Effects of Nel on production of different retinal cell types. Expression constructs for Nel cDNA (A) or artificial miRNA (B) were transfected into the optic vesicle by in ovo electroporation at HH9–11 (E1.5). The numbers of different types of retinal cells in transfected areas were compared with those in corresponding areas transfected with control vectors (EGFP in A, control RNAi in B) at E18. No significant differences were detected in the numbers of AP2α-positive bipolar cells, Pax6-positive horizontal cells, rhodopsin-positive rods, visinin-positive cones, or vimentin-positive Müller glia. In addition, the amacrine cell numbers in the INL were not significantly altered. n = 6 embryos. ANOVA test.