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. 2014 Jan 15;25(2):234–244. doi: 10.1091/mbc.E13-08-0453

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© 2014 Nakamoto et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — Nel promotes survival of retinal cells during development. (A–D) Nel was either overexpressed (A, B) or knocked down (C, D) in half (left side) of the developing retina by in ovo electroporation at HH9–11 (E1.5). Retinal sections were prepared at E6, and apoptotic cells were detected by TUNEL assay (red). The areas of transgene expression are marked by expression of a GFP reporter (transfection side, green). A subset of TUNEL-positive cells is indicated by arrows. Compared to the control side, Nel overexpression decreased the number of TUNEL-positive cells, whereas RNAi knockdown of Nel drastically increased it. nr, neural retina; on, optic nerve. Scale bars, 100 μm. (E) Quantification of apoptotic cells. The number of TUNEL-positive cells on the transfected side was compared with that on the control side and plotted as mean ± SEM. *p < 0.05, **p < 0.0005. n = 6 embryos. ANOVA test.