FIGURE 2:

Acetylation state does not alter the structure of dynamic microtubules. (A) End-on view of the deacetylated, dynamic microtubule reconstruction with the docked electron crystallographic structure of tubulin (1JFF). (B) Inside view (from the microtubule lumen) of the difference maps between acetylated and deacetylated reconstructions contoured at 3σ, with extra density in acetylated microtubules shown in blue and extra density in deacetylated microtubules shown in red. The differences are superimposed on the cryo-EM reconstruction of deacetylated microtubules, shown as gray isosurface. The location of the loop containing the modified residue is indicated by the circle. (C) Outside view, colors as in B. α-Tubulin is shown in green and β-tubulin in blue.