FIGURE 2:

Midbody ring inheritance is asymmetric during male GSC cytokinesis, and the GSC excludes the MR in the GB. (A) An example of a testis apical tip containing a GSC connected to a GB via the MR (Pav-GFP; arrowhead) and a GB containing the MR after complete separation from the GSC (arrow). Ubi-Pav-GFP flies were used. Green, Pavarotti-GFP (Pav-GFP) marking MRs and ring canals; red, FasIII (fasciclin III) marking hub cells and adducin-like (Add) marking the spectrosome/fusome; Blue, Vasa marking germ cells. The asterisk indicates the hub. Bar, 10 μm. (B) An example of a testis apical tip containing two GSC-GB pairs with their MR (arrowheads) inherited by GBs. GSC-GB pairs are indicated by brackets. Ubi-Pav-GFP flies were used. (C) An example of a GB in mitosis, demonstrating that the GB does not contain the MR from the previous mitosis. nos-gal4>UAS-Pav-GFP flies were used. (D) MR observed in CySCs (the attachment of CySCs to the hub is not visible in this focal plane). The inset shows separate channels of Pav-GFP (green) and Add (red). (E) Schematic diagram of MR inheritance during male GSC cytokinesis. nos-gal4>UAS-Pav-GFP flies were used.