FIGURE 3:

MR inheritance is asymmetric during female GSC cytokinesis, and GSCs inherit MR. (A–C) Examples of germaria containing distinct stages of MR inheritance and degradation. The arrowhead in C indicates an MR that lost its ring shape and became smaller. The arrowhead in the inset of C indicates an MR in the round-stage spectrosome before it becomes smaller. Note that the entire spectrosome becomes weakly positive for GFP at this stage. The arrowheads point to MRs, which maintain stronger a GFP signal and appear as yellow in C. Ubi-Pav-GFP flies were used. Green, Pav-GFP; red, FasIII and Add; blue, Vasa. The asterisk indicates cap cells. Bar, 10 μm. (D, E) Selected frames from time-lapse live observation of MR behavior in female GSCs from Pav-GFP flies (from Supplemental Movies S1 and S2). (D) An example of an MR being inherited by the GSC. (E) An example of an MR becoming small at the site of cytokinesis.