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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Vision Res. 2013 Nov 9;94:16–23. doi: 10.1016/j.visres.2013.10.018

Figure 4. Vesl-1/Homer 1 expression is markedly increased in the synaptic layers of the retina.

Figure 4

A) Vesl-1S/Homer 1a immunoreactivity showed a typical expression pattern that did not change in the aged, glaucomatous retina. A representative single confocal section is shown. Scale bar: 20 μm. B) Magnified view of the region in the RGC layer indicated by a square in A). Scale bar: 2.5 μm. C) Quantification using microfluorimetry is presented as the mean intensity of 25 line scans across a length of 180 μm. Intensity of Vesl-1S/Homer 1a immunoreactivity was increased in the synaptic layers, by approx. 60% in the ganglion cell layer (GCL) and the inner plexiform layer (IPL), and approx. 2-fold in the outer plexiform layer (OPL). D) Immunolabeling with an antibody specific for the long Vesl-1L/Homer 1c isoform revealed a significant increase in immunoreactivity in the synaptic layers of the retina of aged DBA/2J mice compared with young controls as evident from a representative single confocal section. Scale bar: 20 μm. E) Magnified view of the region in the RGC layer indicated by a square in D). Scale bar: 2.5 μm. D) The intensity graph represents the mean intensity of 25 line scans across a 180 μm stretch of retina, showing a 3-fold increase in Homer 1c immunoreactivity in the GCL, IPL and OPL.