Table 2.
Equilibrium dissociation constants of wild type and mutant MBNL1 proteins with (CUG)12 and cTNT18 RNA sequences.a
| Protein | (CUG)12 (nM) | cTNT18 (nM) |
|---|---|---|
| Wild type | 150 ± 20 | 1.3 ± 0.2 |
| Phe22Ala | 270 ± 50 | 0.6 ± 0.4 |
| Phe36Ala | 390 ± 20 | 1.4 ± 0.1 |
| Phe54Ala | 510 ± 50 | 2 ± 1 |
| Tyr68Ala | 540 ± 50 | 1.6 ± 0.2 |
| Tyr188Ala | 160 ±10 | 1.3 ± 0.4 |
| Arg195Ala | 180 ± 50 | 1.0 ± 0.4 |
| Arg201Ala | 500 ± 100 | 0.6 ± 0.2 |
| Phe202Ala | 220 ± 50 | 1.3 ± 0.3 |
| Arg231Ala | 190 ± 30 | 1.3 ± 0.2 |
| Tyr236Ala | 70 ± 20 | 0.9 ± 0.3 |
| Wild type – GST | 160 ± 30 | 4 ± 2 |
| ZnF12-GST | NBb | >3750 |
| ZnF34-GST | NB | >5000 |
a
Errors are the standard deviation of at least three independent measurements.
b
NB indicates that no binding was observed in the gel mobility shift assay.