Figure 1.
Expression of the reporter gene LacZ in pig lungs. HD-Ad-K18-LacZ vector particles (5 × 1012) were delivered to the pig's left lung. One week following vector delivery, segments throughout the bronchial tree were collected for X-gal staining. The right lung acted as a negative control without vector delivery. (a) Gross examination of lung segments for X-gal staining. Sections stained blue are from the transduced left lung and a section shown with an arrow is a negative control from untransfected right lung. (b) Microscopic pictures of left main stem bronchus. (c–e) Left bronchiole, small bronchiole, small bronchiole with high magnification. (f) Left lung respiratory bronchiole, showing epithelial specific X-gal staining. (g) Right lung respiratory bronchiole and alveoli, negative for X-gal staining.