Table 1.
Minimum inhibitory concentrations (MIC) for E. coli containing the indicated plasmids
| Drug resistant plasmid Name | Antimicrobial drug | Drug resistance plasmid MIC (ug/ml) | Empty vector MIC (ug/ml) | Fold increase |
|---|---|---|---|---|
| pAMP |
ampicillin |
16 |
4 |
4 |
| pAMP |
piperacillin |
3 |
2 |
1.5 |
| pAMP |
cefotaxime |
<.25 |
<.25 |
0 |
| pAMP |
ceftazidime |
<.5 |
<.5 |
0 |
| pAMP |
cefepime |
0.064 |
0.094 |
0.68 |
| pAMP |
Aztreonam |
0.19 |
0.125 |
1.5 |
| pAMP |
Imipenem |
0.25 |
0.25 |
0 |
| pCIP |
ciprofloxacin |
0.125 |
<0.002 |
>62.5 |
| pCIP |
levofloxacin |
0.38 |
0.012 |
31.7 |
| pCIP |
Ampicillin |
6 |
4 |
1.5 |
| pCIP |
piperacillin |
3 |
2 |
1.5 |
| pCIP |
ceftazidime |
<.5 |
<.5 |
0 |
| pCIP |
cefotaxime |
<.25 |
<.25 |
0 |
| pCIP |
cefepime |
0.125 |
0.094 |
1.3 |
| pCIP |
Imipenem |
0.25 |
0.25 |
0 |
| pTMP |
trimethoprim |
>32 |
0.124 |
>258 |
| pTMP |
trimethoprim- sulfamethoxazole |
0.5 |
0.064 |
7.8 |
| pAZT |
Aztreonam |
12 |
0.125 |
96 |
| pAZT |
cefepime |
1 |
0.094 |
10.6 |
| pAZT |
ceftazidime |
16 |
0.5 |
32 |
| pAZT |
piperacillin |
12 |
2 |
6 |
| pSXT |
trimethoprim-sulfamethoxazole |
1 |
0.064 |
15.6 |
| pSXT |
trimethoprim |
>32 |
0.125 |
>256 |
| pPRP:1B |
ciprofloxacin |
0.125 |
0.016 |
7.8 |
| pPRP:1B | levofloxacin | 0.25 | 0.047 | 5.3 |
MICs in the presence of the plasmid with the metagenomic DNA insert compared to the empty vector (pSMART or 1B) are shown. In accordance with manufactures recommendations, less than a 4-fold increase was considered to be within the margin of error.