Figure 1.

Immunohistochemical analysis of MEKK3 in esophageal tissues. Paraffin-embedded sections of histologically normal mucosa, dysplasia, and ESCC were stained using anti-MEKK3 polyclonal antibody as described in the Methods section. (i) Normal esophageal mucosa showing no MEKK3 immunostaining; (ii) dysplasia depicting nuclear and cytoplasmic MEKK3 immunostaining in epithelial cells; (iii) ESCC illustrating both intense cytoplasmic and nuclear staining in tumor cells; (iv) ESCC section showing cytoplasmic MEKK3 immunostaining; (v) ESCC used as a negative control incubated with isotype specific IgG replacing the primary antibody showing no MEKK3 immunostaining in tumor cells ((i-v) original magnification x 200).