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Examples of intra-epidermal nerve fibres (IENFs) in unoperated (näive) skin. The low-power image (A) shows a strip of control skin that contains several IENFs. Two marked with arrowheads are shown at higher magnification in B and C. Only one of the IENFS shown at high magnification (D) is double labelled for both TrkA (Tropomyosin-related kinase A, blue) and the neuropeptide Calcitonin gene-related peptide (CGRP, red; arrow). Scale bar = 200 μm (A), 10 μm (B, C) and 5 μm (D).
