Flowering function analysis in Arabidopsis and the subcellular localizations of two soybean CO-lineage genes. A and B showed the phenotypes of overexpression of GmCOL2 and 5 in co-2 mutants, respectively. C to H, phenotypes of over-expression of GmFTL1, 2, 3, 4, 5 and 6, respectively. I, total rosette leaf numbers of transgenic lines of GmCOL2 or 5 in co-2 mutants. J, total rosette leaf numbers of transgenic lines of GmFTL1, 2, 3, 4, 5 and 6 at flowering time. n, the total number of tested transgenic lines. Box plot showed total rosette leaf numbers of each line at the beginning of flowering and was generated using GraphPad Prism 5 software. The top of the box is the 75th percentile. The bottom of the box is the 25th percentile. The horizontal line intersecting the box is the median value of the group. Horizontal lines above and below the box represent maximum and minimum values, respectively. Boxes with dissimilar letters are significantly different at P < 0.01 after one-way analysis of variance (ANOVA). K, The subcellular localizations of GmCOL2 and 5 tagged by YFP at the N-terminal in the soybean leaves. PI (Propidium iodide) strain was selected to mark the cell walls.